Chromatographic Fractionation of Deoxyribonucleic Acids
نویسندگان
چکیده
منابع مشابه
Chromatographic fractionation of deoxyribonucleic acids.
The investigation of the chemistry of genetics is facilitated by the availability of homogeneous preparations of deoxyribonucleic acid (DNA). For example, the DNA of T-even bacteriophages, which contain a single molecule of DNA per infectious particle (3), can be extracted as molecules of uniform length (4) and composition (5). Chemical and biological studies of the DNA’s of higher organisms, h...
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The presence of phosphorus-containing protein in the yolk of the hen egg was already recognized in 1900 (1). Since then, the egg yolk phosphoproteins received attention in several laboratories (cf. (2)). The first detailed study of the most highly phosphorylated fraction of yolk phosphoprotein, phosvitin, was reported by Mecham and Olcott (3) in 1949. They isolated a product containing 10% phos...
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Evidence has recently accumulated that histone preparations, particularly those derived from the nuclei of calf thymus, may contain a mixture of different basic proteins. Variations in the amino acid composition of histone fractions extracted and precipitated by different means have been demonstrated by Stedman and Stedman (2), Daly, Mirsky, and Ris (3), Eadie and Leaf (4), Hamer (5), Davison a...
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Guinea pig myelin basic protein has been chromatographed on carboxymethylcellulose at alkaline pH to yield five purified components, each of which was shown to be identical with one of the several components which have previously been observed electrophoretically at alkaline pH. Each purified chromatographic component was stable upon electrophoresis at alkaline pH. No differences in molecular s...
متن کاملChromatographic Studies on Nucleic Acids
The nucleic acids are such universal constituents of biological systems and their function is probably so important that it is not surprising that much labour has been spent on the study of their composition. Until very recently there have been no methods of analysis which could be considered more than semiquantitative, and it now seems doubtful whether many published analyses are even approxim...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1962
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)50144-7